vAL1 sequences
vAL1 sequences

Crystal Structure of Family 14 Polysaccharide Lyase withpH-dependent Modes of Action, JBC 284, 35572 (2009)

Protein, DNA sequences

A561L

vAL1 (S)

pET21b - 5?-GCCATATGACAAATGTTATAAGCACGTTAG-3?
H Met T N V I S T L
pET21b - 5?-CTCGAGTTCGTATTTCTTCATTTGGAAATT-3?
N F Q Met K K Y E L E
VAL1

pET42b - 5?-GGCCATGGATGGAAACGACAACTGGGATA-3?
A Met D G N D N W D
pET42b - 5?-GGGGATCCAATGGAAACGACAACTGGGATA-3?
G S N G N D N W D
Xho1 - CTCGAG
Nde1 - CATATG
Nco1 - CCATGG

psppathsrv2, james, phycodna

GST fusion pGEX - 6P-1

HisTag - pET - 15b