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Summary of lysin activities

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ATCV WAL lysin with umbelliferyl substrates

ATCV WAL lysin with umbelliferyl substrates

Samples contain 75microliters buffer (25mM KOH, 50mM MES, 5mM CaCl₂) 75microliters substrate (25micrograms/ml umbelliferyl derivative) and 2microliters of ATCV WAL lysin

Start



After 90min



Overnight

Conclusions - WAL lysin has phosphatase, beta-D-glucosidase and beta-D-N-acetylglucosaminidase activities. The umbelliferyl acetate is partially hydrolyzed (presumably bad batch)

Assays were photographed with midrange UV illumination through a 520nm long pass filter.