Lysin summary - basics
Lysin summary - basics
Basics of measuring lysin activity - lysin renders chlorophyll detergent accessible
Detergent sensitivity of algal cells (resistant to neutral, anionic detergents)
Intact virion lysins are host specific
Extracted (soluble, WAL) lysin is host nonpecific
Cloned A215L (alginate lyase) has lysin activity
Commercial alginate lyase has lysin activity
High salt dissociates soluble lysin from intact PBCV virions
Improvements in purifying lysin from cell lysates
SDS gel of lysin prep (not very pure)
Cation exchange of soluble lysin - elutes in several fractions, multiple (hetero) subunits
Cation exhange of lysin extracted from virions - elutes at lower salt than lysate lysin
New! - Diethylpyrocarbonate inhibits PBi autolysin
New! - Polyethyleneimine precipitates both DNA and lysin in lysin prep
New! - Antibody to PBCV inhibits WAL lysin assay
New! - Washing cells with 1M LiCl increases sensitivity to lysin
New! - Triton permeability can limit assay sensitivity
New! - Presence of Triton during cation exhange of WAL lysin doesn't affect high MW smear
New! - High MW smear is insensitive to reduction and alkylation
New! - Effect of polymers on the lysin reaction
New! - Effect of sample heating on SDS gels of lysin