Algal virus lysin - summary (extensive summary)
Algal virus lysin - summary
Basics of measuring lysin activity - lysin makes chlorophyll detergent extractable
Detergent sensitivity of algal cells (resistant to neutral, anionic detergents)
PBCV cell wall composition, New! - More compositions - at least 8 sugars
PBCV whole virion lysin - intact virion lysins are host specific
Properties of ATCV WAL lysin (lysin from lysed cells) - host nonspecific
New! - Properties of PBCV lysins
New! - pH and salt dependence of PBCV1 WAL lysin
Calcium ion stabilizes WAL lysin
Effect of inhibitors on lysin (sensitive to thiols, EDTA, proteases, resists iodoacetamide, peroxide, PMSF)
SAG WAL lysin acting on a substrate panel - substrate list (substrates are fluorogenic)
Extracts have beta-NAc-glucosaminidase, alpha-glucosidase and beta-galactosidase activities
Activities of lysed cells - Glycosidases soluble, lysin in wall fraction
Chromatography of PBi soluble fractions, PBCV WAL lysin (independent enzymes)
TCA precipitation of dansyl proteins - Sarkosyl is an effective carrier
Host secretes NAc-beta-glucosaminidase - part of autolysin?
Anion exchange chromatography of PBCV WAL lysin - single component, weak negative charge
Cation exchange chromatography of PBCV WAL lysin - strong positive charge
Lysin assay of cloned A215L - Extracting cloned A215L
Extraction of lysin from PBCV with salt
Effect of alginate lyase on Chlorella strains
Lysin assay with Sybr Gold
Comparative cell specificities of A215L, PBCV1 WAL lysin
Cell specificity of lysin dissociated from PBCV1 with LiCl
Chemical inhibtion of PBCV1 lysin (DEPC, DTT)
Cell specifity of ATCV lysin is the same as that of PBCV1 lysin
Ammonium sulfate treatment of PBCV1
SDS gel of lysin prep
Preliminary secretome
Recent cation exchange
Cation exhange of lysin extracted from virions