Solvents for proteomics
Solvents for proteomics
Solvent M BP r e d ¶d ¶p ¶h Methanol 32.04 64 .792 33 29.7 15.1 12.3 22.3 Ethanol 46.07 78 .785 24.3 26.2 15.8 8.8 19.4 Acetone 58.08 56 .785 20.7 19.7 15.5 10.4 7.0 MEK 72.12 80 .805 18.5 19.3 16.0 9.0 5.1 Ether 74.12 35 .714 4.34 15.4 14.5 2.9 5.1 Tetrahydrofuran 70.09 56 .88 7.52 18.5 16.8 5.7 8.0 Hexane 86.18 69 .66 1.89 14.9 14.9 0 0 Chloroform 119.38 61 1.48 4.8 18.7 17.8 3.1 5.7 Phenol 94.11 182 1.07 9.78 24.1 18.0 5.9 14.9 m-Cresol 108.1 203 1.03 11.5 22.7 18.50 5.1 12.9 Resorcinol (solid) 121.1 277 1.28 3.2 29.0 18.0 8.4 21.1 Toluene 92.14 111 .87 2.4 18.3 18.0 1.4 2.0
r - density
e - dielectric constant
d - Hildebrand solubility parameter
¶d - Hansen dispersion component
¶p - Hansen polar component
¶h - Hansen hydrophilic component
Hexane is miscible with neither water nor phenol
Solubility parameters
Solvent table
Solvent properties
Hydrocarbon boiling pointsOrganic soluble salts
Solvent densities
Ammonium acetate
Trimethylammonium acetate (available as 1M soln. from Sigma)
Organic liquids
Lithium chloride