Qbeta replicase
Qbeta replicase
1- Central dogma
2- RNA viruses circumvent DNA, RNA serves two roles
3- Instability of RNA, small virus genomes
4- Must replicate own RNA
5- Requirements of replicase assay
6- Template specificity
7- ssRNA vs dsRNA as template
8- Importance of template ends
9- Coupling with cell cycle
10- Properties of minivariants (ds structure)
11- Suppressing deletions
12- Competition with translation
13- de novo template synthesis
14- Plaque assay for template
15- Speed 10^10 in 10min with small RQ