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NEB enzyme finder

PCR

Plasmids

vAL1 fasta

A215L fasta

A561L fasta

Primers

Primers

pET21b (vAL1) - 5?-GCCATATGACAAATGTTATAAGCACGTTAG-3?

pET15b (A215L) - 5?-GCCATATGACAAATGTTATAAGCACGTTAG?

pET15b (A561Ls) - 5?-GCCATATGGGAAATGTAAGTGGGACGATAA?

pET15b (A561Ll) - 5?-GCCATATGATGGCCGATGTTGAGGTTCCTA?

pET21b - 5?-CTCGAGTTCGTATTTCTTCATTTGGAAATT-3?

TCATTCGTATTTCTTCATTTGG

pET15b' (A215L) - 5?-GGCTCGAGATTTCTTCATTTGGAAATTCGT-3?