Polyethyleneimine precipitates both lysin and DNA from lysin preps

Polyethyleneimine precipitates both lysin and DNA from lysin preps



2.5 miroliters of neutralized 2.5% PEI were added to 25 microliters of WAL lysin
Cells were centrifuged 5K for 30 sec, a sybr gold pellet was apparent by visual inspection
Attempts to extract lysin from pellet were unsuccessful