Lysin prep #4 - SDS gel
Lysin prep - new protocol. Suspended in (0.25M) pH 4.5 NaAc buffer vs normal pH 7 buffer, insulin, protamine sulfate, RNAse T1 - all alklyated only.
Protamine sulfate gave a visible precipitate that stained red w/ponceau S.
Lysin prep #4 - SDS gel
Lysin prep #4 - SDS gel
Lysin prep - new protocol. Suspended in (0.25M) pH 4.5 NaAc buffer vs normal pH 7 buffer, insulin, protamine sulfate, RNAse T1 - all alklyated only.
Protamine sulfate gave a visible precipitate that stained red w/ponceau S.