Lysin prep - SDS gel
Lysin prepared by the new protocol. Divided into 4 aliquots, all 4 suspended in 25mM KOH, 50mM MOPS. 1,3 and 4 brought to 1M in LiCl. 2 brought to 2M in LiCl. 4ul of 2.5% PEI (polycation) added to 4. 1 and 2 incubated 30 min at 37C; 3 and 4 left at RT. 50ul aliquots pptd. w/TCA. All run on SDS gel.
