Lysin prep - SDS gel
Lysin prepared by the new protocol except half the final pellet taken up in pH 4.5 KAc and the other half in pH 7 K MOPS. LiCl was then added to 1M for each of the two. After suspension of pellets preps were incubated 30 min at 37C and then centrifuged 30 min at 23K in a 50Ti rotor. Supernatants (prufied lysin) were withdrawn and assayed for lysin. In this case TCA ppts were made directly from the LiCl solution w/o dialysis.
A215L (cloned) samples were obtained from Jim Gurnon.
These preps clearly have more virus material than the previous prep. The presumptive lysin band from the previous prep is clearly larger than purified A215L and is only a minor component of this prep. pH 4.5 and pH 7 give slightly different protein spectra.
