Glycosidase activity - lysin preps
NAcglucosaminidase
60microliters MOPS buffer (pH 7), 60microliters substrate - 15microliters lysin, incubated 30min at 50C
+ = lysin activity
++ = high lysin activity
Alpha-glucosidase
Conclusions: Lysin is largely wall associated, glycosidases are soluble
Lysin binds electrostatically to cell walls, glycosidases do not
