NAc-beta-glucosaminidase activity of SAG WAL lysin
NAc-beta-glucosaminidase activity of SAG WAL lysin
100microliters substrate, 100microliters buffer, 2microliters lysin, incubated for 35min @ 50C
Activity is inhibited by calcium (3mM) and relatively resistant to high ammonium concentrations
Effect of temperature on NAc-beta-glucosaminidase activity
500microliters MOPS buffer (pH 7), 500microliters substrate - 5microliters SAG WAL lysin, samples are 120microliter aliquots
N-Ac-beta-glucosaminidase is secreted by Chlorella!