Lysin activity of alginate lyase
Cell suspensions were incubated 1hr with 2ul of commercial alginate lyase (Flavobacterium, 20 mg/ml)
As can be seen the lyase has activity only toward PBi cells. The activity is appreciably higher at pH 8 (Bicine buffer) than at pH 7 (MOPS buffer). It will be worth adding SAG cells (ATCV host) to the substrate collection.
Note the greenish background in the undigested cell suspensions. This is due to intrinsic fluorescence of the enzyme preparation.
