Jovin buffer table
# Buffer MW Sol pK
0oCRel
MobSol pK
25oCRel
Mob84 Sulfate- s 0.00 -1.28 s 0.00 -1.42 97 Potassium+ s 0.00 1.49 s 0.00 1.39 98 Sodium+ s 0.00 1.00 s 0.00 1.00 99 Chloride- s 0.00 -1.63 s 0.00 -1.55 81 Phosphate- 98 s 2.02 -.60 s 2.11 -0.59 1 Alanine 89.09 s 2.47 0.60 s 2.39 0.60 42 Glycine 75.07 s 2.48 0.53 s 2.39 0.68 2 B-alanine 89.09 s 3.69 0.59 s 3.59 0.53 16 Formic acid 46.03 s 3.74 -1.14 s 3.71 -1.06 15 Lactic acid 90.08 s 3.84 -.66 s 3.80 -0.66 34 GABA 103.12 s 4.13 0.64 s 4.07 0.62 18 Acetic acid 60.05 s 4.74 -.0.79 s 4.72 -0.80 19 Propionic acid 74.08 s 4.86 -0.63 s 4.83 -0.70 4 Pyridine 79.10 s 5.50 0.86 s 5.21 0.80 6 Histidine 155.16 0.10 6.35 0.49 0.20 6.04 0.42 5 4-Picoline 93.12 s 6.21 0.71 s 6.06 0.73 35 MES 195.20 0.65 6.41 -0.44 s 6.12 -0.45 20 Cacodylic acid 137.99 s 6.20 -0.53 s 6.21 -0.48 33 Bistris 209.20 s 6.88 0.38 s 6.50 0.42 7 2,6-Lutidine 107.15 s 7.00 0.60 s 6.79 0.56 36 ACES 182.21 0.22 7.34 -0.47 s 6.80 -0.50 # Buffer MW Sol pK
0oCRel
MobSol pK
25oCRel
Mob31 Hexafluoroacetone 184.05 s 6.72 -0.46 s 6.80 -0.48 9 OH-Ethylmorpholine 131.18 s 7.19 0.61 s 6.89 0.57 82 Phosphate-- 0.58 7.07 -0.97 0.60 6.95 -0.96 8 Imidazole 68.08 s 7.46 0.97 s 6.99 0.83 23 TES 229.24 s 7.96 -0.51 s 7.44 -0.48 24 HEPES 238.30 s 7.85 -0.28 s 7.48 -0.28 10 N-Ethylmorpholine 115.18 s 8.03 0.62 s 7.69 0.58 11 Triethanolamine 149.19 s 8.35 0.55 s 7.80 0.55 39 Monotris 165.19 s 8.38 0.35 s 7.83 0.44 26 Veronal 184.19 0.12 8.36 -0.46 0.03 7.94 -0.44 25 Tricine 179.17 0.80 8.60 -0.32 s 8.05 -0.42 12 Tris 121.10 s 8.84 0.41 s 8.07 0.50 27 Glycylglycine 132.12 s 8.94 -0.58 s 8.21 -0.55 32 Bicine 163.20 s 8.74 -0.69 s 8.30 -0.67 13 Morpholine 87.12 s 8.85 0.73 s 8.60 0.76 43 Asparagine 150.12 s 9.00 -0.50 s 8.82 -0.54 38 Ammediol 105.14 s 9.56 0.43 s 8.83 0.50 41 Taurine 125.14 s 9.70 -0.61 s 9.02 -0.63 28 Boric acid 61.84 s 9.46 -0.63 s 9.20 -0.62 83 Ammonia 17 s 10.12 1.52 s 9.29 1.49 14 Ethanolamine 61.08 s 10.35 0.86 s 9.54 0.91 29 Glycine 75.07 s 10.46 -0.74 s 9.74 -0.72 40 GABA 103.12 s 11.33 -0.55 s 10.52 -0.59 30 Proline 115.13 s 11.29 -0.75 s 10.60 -0.75 All measurements at ionic strength 0.01M
Buffer numbers are as assigned in the computer program
from: Jovin, Thomas M., Multiphasic zone electrophoresis IV. Design and analysis of discontinuous buffer systems with a digital computer. Ann NY Acad Sci 209, 477 (1973)