Ideas 8
1- Effect of salt, higher Ts on virion proteins (sharpen slightly)
2- Effect of salt, higher Ts on protease sensitivity of PBCV
3- Effect of incubating virions w/lysin on virion proteins
4- Effect of higher T incubation on lysin proteins
5- Protein composition of different lysin preps
6- Effect of heat on capsid protein in the absence of SDS
7- Can antibodies ppt PBCV from dilute solution (for SDS gel electrophoresis)
8- Develop conditions for proteolysis (minimize protease, inhibit w/PMSF)
9- Sensitivity of lysin to proteolysis
10- Improve lysin purif, LiCl in sucrose gradient, lower LiCl in pellet extraction, acid pH
10a- LiCl extraction series (sucrose pellet)
11- Gel filtration of better lysin preps
12- Wash PBCV w/detergents, high salt
12a- Use sarkosyl, pellet through sucrose pad
13- Deglycosylate capsid peptides
14- What proteins are at top of sucrose gradient?
15- Better gel on capsid peptides
16- Fresh lysin prep
17- Acid purification of PBCV, add increasing amounts of pH 4.5 buffer
18- Add LiCl systematically to PBCV cracking buffer