Ideas6
Ideas7
1- Sr vs Ca as divalent cations - Same
2- Effect of salt conc.
3- Effect of pH (6.7, 7.3)
4- Washed vs unwashed cells - Washed better
5- Proteins released from virion by osmotic shock - No
6- Effect of pH and salt on background lysis
7- Effect of proteases on lysin (chymotrypsin, proteinase K) - assay
8- Effect of DTT on virion stability, stability of lysin activity
9- UV spectrum of lysin
10- Effect of pH on intrinsic stability of cells (vary T)
11- Order primers
12- Effect of various nonionic detergents on lysin kinetics
13- Effects if proteinases as function of salt, pH
14- Effect of T on proteinases
15- Effect of pH on chlorophyll stability