Ideas4
1- Calcium and strontium at lower pH
2- Lysin activity of virion, calcium, EGTA effect, temp series
3- Denature and renature lysin
4- Lysin activity w/Sybr Gold, bleached cells (Clorox, MeOH)
5- Activity through sephadex
6- Sensitivity of virion lysin to protease
7- Does sarkosyl inhibit lysin?
8- Run virion lysin through anion then cation exchange
9- Lysin in secretome??
10- Treat lysins with antibody to whole virus
11- Do thiols, EGTA, Triton help dissociate virus?
12- Is thiol inhibition reversible?
13- Does heat treated virus still have lysin activity?
14- How does EGTA affect LiCl dissociation of PBCV?
15- Does acetone (THF) ppt lysins?
16- Can renaturation kits restore lysin activity?
17- Better picture of antibody effect on WAL lysin
18- Carrier for acetone ppt.
19- Vary KCl w/con KCl soln.
20- Make up basic K-MOPS buffer, adjust pH w/conMOPS soln.
21- Sulfite as cell preservative
22- Effects of PEI, DEAE dextran, dextran sulfate on lysin
23- Effect of organic solvents on lysin