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Protein blast

Prot param

PBCV mutants

PBCV1 genes

PBCV

a561 lysin

Potential glycosylases, lyases

Potential glycosylases, lyases

Basics

All known bacteriophages enter cells using a single enzyme integral to the virion
A215L by its self digests Chlorella walls (a single activity suffices)
Chloroviruses do not produce haloed plaques (characteristic of soluble enzyme release)

Alginate lyases

A215L - 321AA, pI 9.5 (all chloroviruses, N-terminal inhibitory domain)

A561L - 649AA, pI 9.43 (PBCVs, ATCV & virion proteomes, 4 domains!)

N263R - 297AA, pI = 9.79 (PBi analog of A561L, in virion proteome)

"Pectate lyase"

A122/123R - 1033AA, pI=5.3 (vp260 - beta-helix, resembles podovirus tailspike proteins)

Chitinases

A181/182R - 831AA, pI 7.46 (all chloroviruses & C. variabilis) - has chitin binding domain

A260R - 484AA, pI 9.36 (not in all chloroviruses)

Chitosanase

A292L - 328AA, pI 9.04 (all chloroviruses)

Glucanase

A094L - 364AA, pI 4.53 (not in all chloroviruses but in C. variabilis)

A246R - 288AA, pI 10.0, Barwin (endoglucanase) domain, only in PBCVs