Destabilizing PBCV
1- Heat - 40, 50, 55C
2- Higher pH
3- EDTA
4- DTT + Heat
5- DTT + salt
6- Dextran sulfate
7- Cell walls (prepare w/lysin)
8- Osmotic shock
9- Surface protein
10- Lower pH
11- EtOH
Measuring dissociation
1- DNA release (ultracentrifuge, Sybr Gold, antibody, Am sulfate)
2- Sucrose gradient
3- UV
4- Lysin in supernatant
5- Loss of lysin
6- Lysin activity v. nonhost
7- SDS gels of supernatant protein
8- Increased SDS sensitivity
9- Fluorescence microscopy
10- DNase sensitivity of Sybr Gold staining
11- Proteinase K sensitivity in high salt