Cloned a215L, a561L - lysin assay
Cloned a215L, a561L - lysin assay
Assays were 90ul of cells and 10ul of coli suspension. 2's are A215; 3's are A561L
Kinetics of lysin with prep 3bs (A561L). The "S" sample was 3bs supernatant. Activity is clearly in soluble fraction.