A215L (alginate lyase)
A215L (alginate lyase)
"Wall associated lysin" is purified from virus lysates by salt extraction (0.4M KCl) of low speed pellets (presumably containing Chlorella walls).
More recently it is purified from high speed pellets of lysates (which contain walls plus whole virus) by extraction with 1M LiCl. These extracts contain much more DNA (viral) than those of the former method.
>gi|11693126|ref|NP_048562.2| hypothetical protein PBCV1_A215L [Paramecium bursaria Chlorella virus 1]
MNGNDNWDNVVKDYNNLRKNGHDEQETISIIRRKYTDIGPVNQKRLEDQYEKIKPSQKPAPKPAPKTAPKSPPATKNTNV
ISTLDLNLLTKGGGSWNVDGVNMKKSAVTTFDGKRVVKAVYDKNSGTSANPGVGGFSFSAVPDGLNKNAITFAWEVFYPKG
FDFARGGKHGGTFIGHGAASGYQHSKTGASNRIMWQQDGGVIDYIYPPSDLKQKIRGLDPEGHGIGFFEDDFKKALKYDVWN
RIEIGTKMNTFKNGVPQLDGESYVIVNGKKEVLKGIN WSRSPDLVINRFDWNTFFGGPLPSPKNQVAYFTNFQMKKYE
M=35.7, pI=9.5, Cys=0
A561 protein properties