Gel filtration of virus associated lysin (G200)
0.1ml of sample 3 from lysin preps (extracted w/0.4M LiCl w/protamine sulfate (among cleaner preps)).
Lysin assay of fractions:
Blue dextran maximum is in fraction 2, bromphenol blue appearing in the last fractions (above 15)
"S" is the starting material (fraction 3)
Ovalbumin (OA), 45Kd, included as an internal standard (runs too close to major lysin band)
Activity is obviously high MW, correlations to protein bands are ambiguous.
Activity could be multisubunit protein, aggregate, or "smeary" high MW protein.
