Assay of cloned A215L
Incubation was about 60 hrs at RT (Over the weekend)
Note - A215L was inactive in a standard 15 min incubation at 37C. Based on protein amount I would have predicted strong activity with even a one min. incubation assuming identity of A215L and lysin.
+ = induced, A = A215L, - = uninduced, V = Vector alone, Bl = Blank (no additives)
These conditions are comparable to the filter paper disc assays of Sugimoto et al (2000)