E-mail: lclane2(at)gmail.com.
My public home page
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Les Lane E-mail: lclane2(at)gmail.com. My public home page |
My mother made me a scientist without ever intending to. Every other Jewish mother in Brooklyn would ask her child after school, "So? Did you learn anything today?" But not my mother. "Izzy," she would say, "did you ask a good question today?" That difference - asking good questions - made me become a scientist.
Isidor Isaac Rabi
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Plastic microfuge tubes - surface properties, Stabilizing liquid phenol
Detecting fluorescence in gels - - - Journal shortcuts UNL only, bypass IRIS gateway
Peptide mapping with CNBr, Gel electrophoresis tips, Tips for SDS PAGE beginners
Dyes and stains - Qbeta replicase references - Summary - Basics
New! - Densities of cesium and rubidium salts - Densities of cesium chloride solutions
Phenol extraction of proteins - extract leaf proteins, clarify extracts (3rd world friendly)
Viruses online, Proteomics solvents - table and links
Review of phenol extraction - How does it work? - Phenol extraction for proteomics
Ammonium acetate - a convenient, overlooked buffer (3rd world friendly)
Minipurification - "universal" plant virus diagnostic tool, Improved - Deglycosylation of glycoproteins
Nebraska virus diseases, My buffer page, - Crowle's stain - Xylanases
A brief guide to biological detergents, Gluthathione - what is it?
Silver staining protocol for polyacrylamide gels (improved Morrissey)
Heat treatment for plant virus diagnosis (use with minipurification)
dsRNA isolation - useful variants of "Morris & Dodds" - protocol & overview (3rd world friendly)
Eugene Garfield - scientific information pioneer, Creationism - a hobby
Science and the internet - History of sucrose density gradient centrifugation.
Stabilizing and "resurrecting" dithiothreitol stock solutions - 3rd world friendly.
Precipitating proteins and nucleic acids - why waste time dialyzing? (3rd world friendly)
Brome mosaic virus - introduction and properties, Chenopodium quinoa - a "universal" plant virus host
Diagnosing soybean viruses
Calculations for log-linear sucrose gradients (SW56, 60), (SW 65L)
RCF calculations for F14-6x250y rotor
Plant Pathology links, Biochem links
Plant Pathology 864B - course, Biochem 839 - course
Plant Pathology 369L - lab, Foundations 101
Plant Path seminar