Effect of citrate conc. on minipurification

Effect of salt concentration on TSV purfication Effect of salt concentration of BSMV purification

Left panel - tobacco streak minipurified from Chenopodium quinoa. Sarkosyl subsituted for Triton X-100 in 0.6M citrate.

Center panel - note higher citrate concentrations give better yields of barley stripe mosaic

Right panel - note irreproducible contaminants in center lane, these never appear in 0.4M citrate, darkness at top of gel is probably running buffer contaminants