Qbeta replicase

Qbeta replicase

1- Central dogma

2- RNA viruses circumvent DNA, RNA serves two roles

3- Instability of RNA, small virus genomes

4- Must replicate own RNA

5- Requirements of replicase assay

6- Template specificity

7- ssRNA vs dsRNA as template

8- Importance of template ends

9- Coupling with cell cycle

10- Properties of minivariants (ds structure)

11- Suppressing deletions

12- Competition with translation

13- de novo template synthesis

14- Plaque assay for template

15- Speed 10^10 in 10min with small RQ