Qbeta replicase
Qbeta replicase
1- Requirements of replicase assay
2- Template specificity
3- ssRNA vs dsRNA as template
4- Importance of template ends
5- Coupling with cell cycle
6- Properties of minivariants (ds structure)
7- Suppressing deletions
8- Competition with translation
9- de novo template synthesis
10- Plaque assay for template
11- Speed 10^10 in 10min with small RQ