Effect of pH on phenol extraction

Effect of pH on protein phenol extraction

Sample (tobacco with TMGMV, STMV) is smeary w/o added base

Note bands near top of gel that disappear as more phenol is neutralized, These are likely DNA (See Pusztai, Biochem J 99,93)

NEM (N-ethylmorpholine) is essentially a liquid (convenient) equivalent of tris.