Minipurification vs. total proteins

Minipurification vs. total proteins

Samples minipurified with 0.4M citrate, each has virus from about 3mg of tissue

Total proteins obtained by phenol extraction - each lane represents about 50g tissue

Capsid proteins cannot be seen among total proteins, however WSMV CI protein (~64K) is visible!

Note rubisco L and rubisco S in all samples