Detergent sensitivity of NC46a cells

Detergent sensitivity of NC46a cells

Cells were incubated 30 min at 37C and centrifuged 30sec at 5K. Released chlorophyll appears as red fluorescence

Triton is 1% Triton X-100 (neutral)
Sarkosyl is 1% N-Lauroylsarcosinate (anionic)
SDS is 1% sodium dodecyl sulfate (strong anionic)
CTAB is 0.5% cetyltrimethylammonium bromide (cationic)
CPC is 0.5% cetylpyridinium chloride (cationic)

Cationic detergents extract chorophyll from Chlorella cells

Cells are resistant to Triton and SDS at least up to 70C

Cells are resistant to acidic pHs