Glycosidase activity - lysin preps

Glycosidase activity - lysin preps

NAcglucosaminidase

60microliters MOPS buffer (pH 7), 60microliters substrate - 15microliters lysin, incubated 30min at 50C

+ = lysin activity
++ = high lysin activity

Alpha-glucosidase



Conclusions: Lysin is largely wall associated, glycosidases are soluble
Lysin binds electrostatically to cell walls, glycosidases do not