Laemmli buffer recipes
Laemmli buffer recipes - weigh and pour
4X conc. resolving gel buffer*
1.5M Tris, pH ~ 8.7
8.8ml con HCl
4X conc. stacking gel buffer**
0.5M Tris, pH 6.8
7.8g Tris HCl
10x Well Buffer
Be aware of why adding liquid HCl is superior to adjusting with a pH meter
*If you're interested in large proteins, more HCl will give better stacking. If you're interested in small proteins, less HCl will release more proteins from the "stack".
**If you understand why I don't use liquid HCl here, give yourself extra credit for understanding buffers.