Secretome ideas

Secretome ideas

1- Concentrate 500ml ov culture to 20ml, then incubate overnight

2- TCA ppt of supernatant

3- Phenol extraction of supernatant

4- Ammonium sulfate ppt of supernatant

5- Develop dansyl insulin as an internal standard

6- SDS extraction of cells

7- Effect of various media on secretome

8- Lysin activity in secretome?