1- Concentrate 500ml ov culture to 20ml, then incubate overnight
2- TCA ppt of supernatant
3- Phenol extraction of supernatant
4- Ammonium sulfate ppt of supernatant
5- Develop dansyl insulin as an internal standard
6- SDS extraction of cells
7- Effect of various media on secretome
8- Lysin activity in secretome?