Effect of DTT quality on reduction-alkylation
Left - reduced and alklyated standards Left - DTT reduced with tributylphosphine Right - standards alkylated only Right - DTT from the bottle
Standards are BSA. Rabbit muscle aldolase, carbonic anhydrase, and tobacco mosaic capsid
Samples reduced with DTT, alkylated with iodoacetamide
Note that reduction-alkylation dramatically reduces mobility of BSA.