Effect of DTT quality on reduction-alkylation

Effect of DTT reduction on protein mobility How bad DTT affects protein mobility
Left - reduced and alklyated standards Left - DTT reduced with tributylphosphine
Right - standards alkylated only Right - DTT from the bottle

Standards are BSA. Rabbit muscle aldolase, carbonic anhydrase, and tobacco mosaic capsid

Samples reduced with DTT, alkylated with iodoacetamide

Note that reduction-alkylation dramatically reduces mobility of BSA.