Cracking buffer recipe
10% (w/w) sodium dodecyl sulfate (SDS) 5.0ml 2% 0.1% crystal violet 1.0ml 0.004% Ficoll 2.5g 10% Tris (3M, pH 8.8)
(36.3g Tris + 3.7ml con HCl + 68.9ml H2O)0.5ml 0.06M 0.1M Na3EDTA 0.5ml 0.002M distilled water 16.0ml
Borate cracking buffer - no reactive amines
10% (w/w) sodium dodecyl sulfate (SDS) 5.0ml 2% 0.1% crystal violet 1.0ml 0.004% Ficoll 2.5g 10% 0.1M Borax 5ml 0.02M 0.1M Na3EDTA 0.5ml 0.002M distilled water 10.0ml