Maize virus minipurifications
Maize virus minipurifications

corn virus minipurifications

Note - corn is a relatively "clean" tissue and also a C3 plant with less rubisco than C4s
Note - see how trypsin "shaves" the large subunit of rubisco as well as WSMVcp and MDMVcp, lanes 5,6
Note - WSMVcp is partially cleaved (common in older infections) even in standard extract, lanes 3,9
Note - partial degradation of rubisco L and WSMVcp when DTT is in the extraction buffer, lane 8