Coomassie Blue staining (Wang et al)
from Wang,X., Li,X., Li,Y. Biotech Lett. 29, 1599-1603 (2007)
1- Fix 1hr in 10% HOAc, 10% MeOH, 40% EtOH (20vol)
2- Sensitize 2hrs in 1% HOAc, 10% ammonium sulfate
3- Stain 4hrs to overnight in 5% HOAc, 45% EtOH, 0.125% CBB R
4- Destain 1hr in 5% HOAc, 40% EtOH
5- Destain until clear in 3% HOAc, 30%EtOH
5- Store in 5% HOAc