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Coomassie Blue staining
Coomassie Blue staining

Staining solution

1g Coomassie Brilliant Blue R250
455ml methanol (Tech)
455ml distilled Water
90ml glacial acetic acid

1- For Coomassie Blue staining, soak about 30min at 50oC with shaking in staining solution. The staining solution may be reused, but its quality gradually deteriorates. Filter staining solution initially and after each use.

Destaining solution

700ml methanol
200ml glacial acetic acid
dilute to 3.5 liters with distilled water