Cation exchange - PBCV1 WAL lysin - 2
Sample 5 (0.65M LiCl wash) was diluted 5 fold, and applied to Acrosep S column equilibrated w/0.2M NH4Ac. The column was eluted with 0.2, 0.4, 0.6, and 1.0 M NH4Ac.
Activity elutes at 0.6, 1.0 ammonium acetate, i.e strongly bound
Fractions were precipitated with TCA, dissolved in 25 microliters of borate cracking buffer containing 2mM iodoacetamide, heated 4 min at 100C. Aliquots were applied to 4-20% polyacrylamide gels (SDS). Gels were stained with silver
Note - "S" is starting material
Does lysin have multiple subunits?
Note the smear of DNA above the protein bands.
