Sample exam
ipage
Les Lane

E-mail: llane1@unl.edu.

My public home page

My mother made me a scientist without ever intending to. Every other Jewish mother in Brooklyn would ask her child after school, "So? Did you learn anything today?" But not my mother. "Izzy," she would say, "did you ask a good question today?" That difference - asking good questions - made me become a scientist.
Isidor Isaac Rabi

Can't find what you're looking for? Do a domain specific Google search.
In advanced search, enter your search terms and plug "lclane.net" into the domain box (lowest on the page).

Plastic microfuge tubes - surface properties, Stabilizing liquid phenol

Detecting fluorescence in gels - - - Journal shortcuts UNL only, bypass IRIS gateway

Peptide mapping with CNBr, Gel electrophoresis tips, Tips for SDS PAGE beginners

Dyes and stains - Qbeta replicase references - Summary - Basics

New! - Densities of cesium and rubidium salts - Densities of cesium chloride solutions

Phenol extraction of proteins - extract leaf proteins, clarify extracts (3rd world friendly)

Viruses online, Proteomics solvents - table and links

Review of phenol extraction - How does it work? - Phenol extraction for proteomics

Ammonium acetate - a convenient, overlooked buffer (3rd world friendly)

Minipurification - "universal" plant virus diagnostic tool, Improved - Deglycosylation of glycoproteins

Nebraska virus diseases, My buffer page, - Crowle's stain - Xylanases

A brief guide to biological detergents, Gluthathione - what is it?

Silver staining protocol for polyacrylamide gels (improved Morrissey)

Heat treatment
for plant virus diagnosis (use with minipurification)

dsRNA isolation - useful variants of "Morris & Dodds" - protocol & overview (3rd world friendly)

Eugene Garfield - scientific information pioneer, Creationism - a hobby

Science and the internet - History of sucrose density gradient centrifugation.

Stabilizing and "resurrecting" dithiothreitol stock solutions - 3rd world friendly.

Precipitating proteins and nucleic acids - why waste time dialyzing? (3rd world friendly)

Brome mosaic virus - introduction and properties, Chenopodium quinoa - a "universal" plant virus host

Diagnosing soybean viruses

Calculations for log-linear sucrose gradients (SW56, 60), (SW 65L)

RCF calculations for F14-6x250y rotor

Plant Pathology links, Biochem links

Plant Pathology 864B - course, Biochem 839 - course

Plant Pathology 369L - lab, Foundations 101

Plant Path seminar